Comparison of soy and meat peptones as ingredients of Clostridium chauvoei culture medium and their effect on immunogenicity and efficacy of the inactivated cultures
2025
L. Abdolmohammadi Khiav | A. Zahmatkesh | B. Majidi | A. Emadi
In immunised hosts, peptone from meat or animal sources may cause prion diseases or contain anti-genic peptides that potentially trigger anaphylactic and other adverse immune reactions. Therefore, soy peptone (SP) was evaluated as a potential substitute for meat peptone (MP) in the culture me-dium. C. chauvoei was inoculated into it to determine the optimal formulation based on its maximum growth. Following this, C. chauvoei was inoculated on both SP and MP culture media, subsequently evaluating their immunogenicity and efficacy. Based on our results, the SP (“C” formulation) pro-vided more suitable conditions than the MP for growth in a shorter time. The number of bacteria was 28×106 units/mL and 19×106 units/mL for SP and MP, respectively, and their difference between two groups was statistically significant (P<0.01). Alpha-toxin amount in MP and SP media was calculated as 134 IU/mL and 155 IU/mL, respectively. The pathogenicity test results in the MP and SP media were 1:90 and 1:100 mL/guinea pig, and did not differ statistically significantly (P>0.01). The results of potency tests showed that the vaccinated guinea pigs were resistant against the (minimum lethal dose) 4MLD challenge against the highly pathogenic strain. According to ELISA results, the amount of antibody in the SP was slightly higher than the MP medium. Statistical analysis indicated that there was no significant difference between two groups of the media (P>0.01). Our results suggest that the SP media can replace the MP. However, immunisation of more animals for evaluation of SP in vivo assays is suggested.
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