The crucial role of CFAS in maintaining acid-stress homeostasis of Hypsizygus marmoreus
2025
Tianyu Huang | Gen Zou | Lihua Tang | Erzheng Su | Fuhou Li | Dapeng Bao | Yu Cao | Ming Gong
Maintaining a stable and neutral pH level in the soil is of great significance for improving soil quality, particularly in acidic soils. Hypsizygus marmoreus, a widely cultivated edible fungus with high nutritional value, exhibits a strong ability to maintain homeostasis under acid stress conditions. This inherent trait positions it as a promising candidate for the bioremediation of acidic soils, thereby highlighting the urgent need to elucidate the mechanisms underlying its acid stress homeostasis. This study shows that low-concentration citric acid stimulates protein metabolism, optimizes substrate supply, and consequently promotes mycelial growth. High concentration citric acid leads to a metabolic shift towards the production of soluble proteins, driven by the enhanced lipid metabolism. Stress experiments prove heterologous expression of H. marmoreus cyclopropane-fatty-acyl-phospholipid synthase (CFAS) increases yeast colony density under citric acid and cadmium stress. Physiological indicator detection shows that CFAS expression raises ATP, mitochondrial H+ -ATPase activity, and soluble protein in yeast, while reducing malondialdehyde (MDA) and reactive oxygen species (ROS). Transcriptomics confirms it enhances lipid and mitochondrial energy metabolism. These findings highlight H. marmoreus CFAS's role in acid-stress homeostasis and the potential of its mycelia in spent cultivation substrate to ameliorate acidic soils.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Directory of Open Access Journals