AFLP diversity among selected foliar diseases resistant groundnut (Arachis hypogaea L.) germplasm
2002
Dwivedi, S L | Gurtu, S | Nigam, S N
Knowledge of genetic diversity facilitiates the judicious use of germplasm in crop improvement programs. The amplified fragment length polymorphism (AFLP) technique was employed to identify foliar diseases resistant accessions with distinct DNA profiles for mapping and genetic enhancement in groundnut (Arachis hypogaea L.). This study revealed 93.3% polymorphic AFLP fragments and 7.2 unique AFLP markers per primer pair. Primer pairs E-ACA + M-CAG, E-AAC + M-CAT, E-ACA + M-CTG, E-AAC + M-CTT, and E-ACC + M-CTC detected between 11-12 unique markers. Accessions with distinct DNA profiles (ICG 6284 with ICGV 99001; TMV 2 with ICG 405, ICGV 1705, ICG 6284, ICGV 99004, and ICGV 99005) and with more number of unique markers (ICG 405, ICG 1705, ICG 6284, ICGV 9904, and ICGV 99005) were identified to diversify breeding populations and identify AFLP markers/quantitative trait loci linked with resistance to ELS, LLS, and rust in groundnut.
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