Plasmid transformation of homofermentative lactobacilli isolated from grass silage and cloning of bacterial chitinase into lactic acid bacteria
1991
Brurberg, M.B. (Agricultural Univ. of Norway, As-NLH (Norway). Dept. of Animal Science) | Nes, I.F. | Sundheim, L.
Lactic acid bacteria are widely used as silage additives. Modern gene technology has introduced a way of producing strains with traits important to good silage making. We have decided to clone a chitinase coding gene, which is known to possess antifungal properties, into lactic acid bacteria. Chitin is a major component of the cell walls of fungi, and the production and secretion of chitinases may be important in the biological control of fungi. This cloning will also serve as a model system for heterologous gene expression in lactic acid bacteria. The development of improved lactic acid bacteria for ensilage depends heavily upon the ability to introduce DNA molecules into the organisms by transformation. Our project started with development of a plasmid transformation systems for homofermentative lactobacilli isolated from grass ensilage.
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