Aseptic dual culture of the greenhouse whitefly, Trialeurodes vaporariorum Westw. (Hom., Aleyrodidae) and its host, Stellaria media (L.) Vill. (Caryophyllaceae)
1992
Raman, A. (Heidelberg Univ. (Germany). Botanisches Inst.) | Beiderbeck, R.
After surface-sterilization by a simple technique the greenhouse whitefly Trialeurodes vaporariorum was cultivated in aseptic dual cultures with calluses or micropropagated shoots of diffferent plant species. Callus cultures from either host plants or non-host plants were never acceptable to the whitefly either for nutrition or for oviposition. On shoot cultures of the host Stellaria media the life cycle of the whitefly was completed and repeated. This dual culture enables the study of the biology of a phytophagous insect under completely controlled and quarantined conditions requiring very limited shelf space
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