A microwell cytotoxicity assay using Artemia salina (brine shrimp)
1993
Solis, P.N. (London Univ. (United Kingdom). School of Pharmacy. Dept. of Pharmacognosy) | Wright, C.W. | Anderson, M.M. | Gupta, M.P. | Phillipson, J.D.
A new microplate assay for cytotoxicity testing using Artemia salina has been developed and shown to give results comparable to a previously published testtube method. The assay reliably detected all of the compounds toxic to KB cells in a series of 21 pharmacologically active agents, except for two which require metabolic activation in man. Four quassinoids with cytotoxic and antiplasmodial activity were also toxic to the brine shrimp while quassin itself was inactive in all three systems. It is proposed that this assay provides a convenient means by which the presence of cytotoxic quassinoids may be detected during the fractionation of plant extracts.
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