Chloroplast genome organization of bromegrass, Bromus inermis Leyss
1993
Pillay, M. (Washington State Univ., Prosser (USA). Dept. of Crop and Soil Sciences. Irrigated Agriculture Research and Extension Center)
A physical map of the Bromus inermis chloroplast genome was constructed using heterologous probes of barley and wheat chloroplast DNA (cpDNA) to locate restriction sites. The map was aligned from data obtained from filter hybridization experiments on single and double enzyme digests. Cleavage sites for the enzymes Pst1, Sal1, Kpn1, Xho1 and Pvu2 ware mapped. The chloroplast genome of B. inermis is similar in physical organization to that of other grasses. The circular cpDNA molecule of B. inermis has the typical small (12,8 kbp) and large (81,3 kbp) single-copy regions separated by a pair of inverted repeat (21 kbp) regions. The cpDNA molecule of B. inermis is collinear in sequence to that of wheat, rye, barley and oats. No structural rearrangements or major deletions were observed, indicating that the cpDNA of Bromus is a useful tool in phylogenetic studies.
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