Effect of duration of culture of grape (Vitis vinifera L.) microcuttings on medium with zeatin riboside on shoot tip recovery after cryopreservation
2012
MARKOVIC , Zvjezdana (INRA , Montpellier (France). UMR 1334 Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales) | Chatelet , Philippe (INRA , Montpellier (France). UMR 1334 Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales) | Sylvestre , Isabelle (Institut de Recherche pour le Développement, Montpellier(France). UMR DIADE) | Karoglan-Kontić , Jasminka (University of Zagreb, Zagreb(Croatie). Faculty of Agriculture) | Engelmann , Florent (Institut de Recherche pour le DéveloppementBioversity International, MontpellierRome(France). UMR DIADE)
Cryopreservation is the only method currently available for the safe, cost-effective, long-term conservation of vegetatively propagated plant species such as grapevine. Cryopreservation protocols have been established for shoot tips sampled from grapevine in vitro plantlets (Plessis et al 1993; Wang et al 2000; Zhao et al 2001; Matsumoto and Sakai 2003). An important parameter for successful cryopreservation is the physiological state of the plant material, and buds taken at different levels on the shoots of the stock plants could differ in this regard. It can then be advisable to preculture microcuttings to obtain axillary shoots from which terminal buds can be sampled, constituting a more homogenous explant population. A cytokinin can be used at this stage to achieve quicker and more homogenous shooting from the microcuttings. Zeatin riboside (ZR) has been shown to stimulate bud proliferation in grapevine (Goussard 1987). As culture duration on ZR medium could affect the physiological state of the plant material, we investigated the effect of this parameter on grape shoot tip recovery after cryopreservation.
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