Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain

Bréard , Emmanuel
        INRA
         .
         UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie; Sailleau , Corinne
        INRA
         .
         UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie; Coupier , Hervé
        . Mérial Grande Prophylaxis Enterprise; Mure-Ravaud , Karine
        . Mérial Grande Prophylaxis Enterprise; Hammoumi , Saliha
        INRA
         .
         UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie; Gicquel , Bernard
        INRA
         .
         UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie; Hamblin , Chris
        . Pirbright Laboratory; Dubourget , Philippe
        . Mérial Grande Prophylaxis Enterprise; Zientara , Stephan
        INRA
         .
         UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie

Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain

2003

Bréard , Emmanuel (INRA (France). UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie) | Sailleau , Corinne (INRA (France). UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie) | Coupier , Hervé (Sanofi, Lyon(France). Mérial Grande Prophylaxis Enterprise) | Mure-Ravaud , Karine (Sanofi, Lyon(France). Mérial Grande Prophylaxis Enterprise) | Hammoumi , Saliha (INRA (France). UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie) | Gicquel , Bernard (INRA (France). UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie) | Hamblin , Chris (Institute for Animal Health, Pirbright(Royaume Uni). Pirbright Laboratory) | Dubourget , Philippe (Sanofi, Lyon(France). Mérial Grande Prophylaxis Enterprise) | Zientara , Stephan (INRA (France). UMR 1161 UMR INRA / ENV Alfort / AFSSA : Virologie)

Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.

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