Genomic approach to study floral development genes in rosa sp.
2011
Dubois , Annick (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Remay , Arnaud (INRA (France). UMR 1259 Génétique et Horticulture) | Raymond , Olivier (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Balzergue , Sandrine (INRA , Evry (France). UMR 1165 Génomique Végétale) | Chauvet , Aurélie (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Maene , Marion (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Pecrix , Yann (Institut Méditerranéen d'Ecologie et de Paléoécologie, Marseille(France).) | Yang , Shu-Hua (Institut National de la Recherche Agronomique, Lyon(France). UMR 0879 Reproduction et Développement des Plantes. RDP, (CNRS / ENSL / U. Lyon 1), Centre de recherche de Clermont-Ferrand-Theix. Biologie et Amélioration des Plantes ) | Jeauffre , Julien (INRA (France). UMR 1259 Génétique et Horticulture) | Thouroude , Tatiana (INRA (France). UMR 1259 Génétique et Horticulture) | Boltz , Veronique (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Martin-Magniette , Marie-Laure (INRA , Evry (France). UMR 1165 Génomique Végétale) | Janczarski , Stéphane (Institut National de la Recherche Agronomique, Lyon(France). UMR 0879 Reproduction et Développement des Plantes. RDP, (CNRS / ENSL / U. Lyon 1), Centre de recherche de Clermont-Ferrand-Theix. Biologie et Amélioration des Plantes ) | Legeai , Fabrice (INRA , Le Rheu (France). UMR 1099 Biologie des Organismes et des Populations Appliquée à la Protection des Plantes) | Renou , Jean-Pierre (INRA , Evry (France). UMRUMR 11651259 Génomique VégétaleGénétique et Horticulture) | Vergne , Philippe (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes) | Le Bris , Manuel (Institut Méditerranéen d'Ecologie et de Paléoécologie, Marseille(France).) | Foucher , Fabrice (INRA (France). UMR 1259 Génétique et Horticulture) | Bendahmane , Mohammed(auteur de correspondance) (INRA , Lyon (France). UMR 0879 Reproduction et Développement des Plantes)
Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower
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