Contribution of a PerR-like regulator to the oxidative-stress response and virulence of Enterococcus faecalis

Verneuil , Nicolas
        INRA
        , Caen .
         USC 2017 Unité sous contrat microbiologie de l'environnement; Rincé , Alain
        INRA
        , Caen .
         USC 2017 Unité sous contrat microbiologie de l'environnement; Sanguinetti , Maurizio
        . Institute of Microbiology - Istituto de Microbiologia; Posteraro , Brunella
        . Institute of Microbiology - Istituto de Microbiologia; Fadda , Giovanni
        . Institute of Microbiology - Istituto de Microbiologia; Auffray , Yannick
        INRA
        , Nantes .
         USC 2013 Unité sous contrat Laboratoire d'étude des résidus et contaminants dans les aliments; Hartke , Axel
        INRA
        , Caen .
         USC 2017 Unité sous contrat microbiologie de l'environnement; Giard , Jean-Christophe
        INRA
        , Caen .
         USC 2017 Unité sous contrat microbiologie de l'environnement

Contribution of a PerR-like regulator to the oxidative-stress response and virulence of Enterococcus faecalis

2005

Verneuil , Nicolas (INRA , Caen (France). USC 2017 Unité sous contrat microbiologie de l'environnement) | Rincé , Alain (INRA , Caen (France). USC 2017 Unité sous contrat microbiologie de l'environnement) | Sanguinetti , Maurizio (Università Cattolica del Sacro Cuore, Rome(Italie). Institute of Microbiology - Istituto de Microbiologia) | Posteraro , Brunella (Università Cattolica del Sacro Cuore, Rome(Italie). Institute of Microbiology - Istituto de Microbiologia) | Fadda , Giovanni (Università Cattolica del Sacro Cuore, Rome(Italie). Institute of Microbiology - Istituto de Microbiologia) | Auffray , Yannick (INRA , Nantes (France). USC 2013 Unité sous contrat Laboratoire d'étude des résidus et contaminants dans les aliments) | Hartke , Axel (INRA , Caen (France). USC 2017 Unité sous contrat microbiologie de l'environnement) | Giard , Jean-Christophe (INRA , Caen (France). USC 2017 Unité sous contrat microbiologie de l'environnement)

PerR is one of the most important transcriptional regulators involved in the oxidative-stress response in Bacillus subtilis. Here, the homologous gene in Enterococcus faecalis, ranked among the leading causes of nosocomial infection, was characterized and analysed. Phenotype analysis showed that the perR mutant was significantly more resistant to H2O2 challenge (P<0·05). Expression of eight genes with potential roles in the oxidative-stress response was determined in the wild-type and perR-mutant strains by real-time quantitative PCR. Surprisingly, low quantitative differences in the transcriptional activity of these genes in the mutant versus wild-type were observed. Likewise, this locus was not involved in survival within murine macrophages, but in the mouse peritonitis model, the perR mutant appeared less lethal than the JH2-2 wild-type strain. The combined results show that PerR affects E. faecalis virulence and that its implication in the transcriptional regulation in this bacterium deviates from the B. subtilis model.

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