In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoterIn vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoter
2001
Coutos-Thévenot , Pierre (INRA , BEAUCOUZE (France). UMR 0077 Pathologie Végétale : Biodiversité, Ecologie, Interactions Bioagresseurs/Plantes) | Poinssot , Benoît (INRA , Dijon (France). UMR 1347 Agroécologie) | Bonomelli , A. (Moët et Chandon, Epernay(France). LVMH-Recherche) | Yean , H. (Moët et Chandon, Epernay(France). LVMH-Recherche) | Breda , C. (Centre National de la Recherche Scientifique, Gif-sur-Yvette(France). UPR2355 Institut des sciences du végétal (ISV)) | Buffard , D. (Centre National de la Recherche Scientifique, Gif-sur-Yvette(France). UPR2355 Institut des sciences du végétal (ISV)) | Esnault , R. (Centre National de la Recherche Scientifique, Gif-sur-Yvette(France). UPR2355 Institut des sciences du végétal (ISV)) | Hain , R. (Bayer AG, Leverkusen(Allemagne). Institut für Biotechnologie) | Boulay , M. (Moët et Chandon, Epernay(France). LVMH-Recherche)
Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening, A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock, Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic control, but others accumulated resveratrol from 5-100-fold, Moreover, in the latter clones, symptoms were highly reduced in response to infection, These results were a good indication that the combination of a pathogen-inducible promoter and a defence gene may increase tolerance against fungi in grapevine, The efficacy of this approach should be further tested by experiments conducted in the vineyard.Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening, A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock, Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic control, but others accumulated resveratrol from 5-100-fold, Moreover, in the latter clones, symptoms were highly reduced in response to infection, These results were a good indication that the combination of a pathogen-inducible promoter and a defence gene may increase tolerance against fungi in grapevine, The efficacy of this approach should be further tested by experiments conducted in the vineyard.
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