Transcriptomics of actinorhizal symbioses reveals homologs of the whole common symbiotic signaling cascade
2011
Hocher , Valérie (INRA , Montpellier (France). UMR 1334 Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales) | Alloisio , Nicole (INRA , Villeurbanne (France). USC 1193 USC INRA / CNRS / Univ. de Lyon : Ecologie Microbienne de Lyon) | Auguy , Florence (Institut de Recherche pour le Développement(France). Equipe Rhizogenèse, UMR Diversité Adaptation et Développement des Plantes) | Fournier , Pascale (Université Lyon 1(France). Centre National de la Recherche Scientifique, UMR 5557, Ecologie Microbienne) | Doumas , Patrick (INRA , Montpellier (France). UMR 0386 Biochimie et Physiologie Moléculaire des Plantes) | Pujic , Petar (Université Lyon 1(France). Centre National de la Recherche Scientifique, UMR 5557, Ecologie Microbienne) | Gherbi , Hassen (INRA , Montpellier (France). UMR 1334 Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales) | Queiroux , Clothilde (Université Lyon 1(France). Centre National de la Recherche Scientifique, UMR 5557, Ecologie Microbienne) | Da Silva , Corinne (Commissariat à l'Energie Atomique et aux Energies Alternatives(France). Direction des Sciences du Vivant, Institut de Génomique, Genoscope) | Wincker , Patrick (Commissariat à l'Energie Atomique et aux Energies Alternatives(France). Direction des Sciences du Vivant, Institut de Génomique, Genoscope) | Normand , Philippe (Université Lyon 1(France). Centre National de la Recherche Scientifique, UMR 5557, Ecologie Microbienne) | Bogusz , Didier (Institut de Recherche pour le Développement(France). UMR Diversité Adaptation et Développement des Plantes)
Comparative transcriptomics of two actinorhizal symbiotic plants, Casuarina glauca and Alnus glutinosa, was used to gain insight into their symbiotic programs triggered following contact with the nitrogen-fixing actinobacterium Frankia. Approximately 14,000 unigenes were recovered in roots and 3-week-old nodules of each of the two species. A transcriptomic array was designed to monitor changes in expression levels between roots and nodules, enabling the identification of up-and downregulated genes as well as root-and nodule-specific genes. The expression levels of several genes emblematic of symbiosis were confirmed by quantitative polymerase chain reaction. As expected, several genes related to carbon and nitrogen exchange, defense against pathogens, or stress resistance were strongly regulated. Furthermore, homolog genes of the common and nodule-specific signaling pathways known in legumes were identified in the two actinorhizal symbiotic plants. The conservation of the host plant signaling pathway is all the more surprising in light of the lack of canonical nod genes in the genomes of its bacterial symbiont, Frankia. The evolutionary pattern emerging from these studies reinforces the hypothesis of a common genetic ancestor of the Fabid (Eurosid I) nodulating clade with a genetic predisposition for nodulation.
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