Precautions for the structural analysis of the gelatinous layer in tension wood
2005
Clair , Bruno (INRA (France). UMR 0745 UMR INRA / CIRAD / ENGRE / CNRS : Ecologie des Forêts de Guyane) | Gril , Joseph (Université Montpellier 2, Montpellier(France). UMR 5508 Laboratoire de mécanique et génie civil (LMGC)) | Baba , kei'ichi (Kyoto University, Kyoto(Japon). Research institute for sustainable humanosphère. Laboratory of biomass morphogenesis and information) | Thibaut , Bernard (INRA (France). UMR 0745 UMR INRA / CIRAD / ENGRE / CNRS : Ecologie des Forêts de Guyane) | Sugiyama , Junji (Kyoto University, Kyoto(Japon). Research institute for sustainable humanosphère. Laboratory of biomass morphogenesis and information)
The gelatinous layer (G-layer) of tension wood fibres in hardwood contributes to the mechanical function of the living tree and has significant consequences on properties of solid wood. Its size, shape and structure observed by optical or electron microscopy exhibits characteristic anatomical features. However, we found that sectioning of non-embedded wood samples results in an uncontrolled swelling of the G-layer was monitored by serial sections, from an embedded wood sample, from its trimmed transverse face to that located several hundreds of micrometers deep. The results revealed that the initial cutting before embedding produced a border effect responsible for the swollen nature, which is similar to sections from non-embedded material. After a conventional embedding technique was applied, a section of at least 30 micrometres below the trimming surface is required to observe an un-swollen G-layer.
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