Quantifying cell polarities in confocal images using 3D wall meshes
2019
Cerutti, Guillaume | Galvan Ampudia, Carlos | Legrand, Jonathan | Godin, Christophe | Vernoux, Teva
Polarized transport of signaling molecules, such as the phytohormone auxin, is a core process for the establishment of gradients involved in the patterning of multicellular plant tissues. Cell-to-cell polarity of transport can result from the differential deposition of efflux carriers on the membranes at the interfacebetween cells, as for the pin-formed1 (PIN1) auxin transporter. Quantifying such sub-cellular information in whole organ microscopy images constitutes a real challenge. In this work, we propose a method that computes polarities at the level of cell-to-cell interfaces starting from standard resolution confocalimages and using a 3D geometric representation of cell walls. A robust estimation of spatial fluorescence distribution around cell walls allows quantifying polarity with a fair level of confidence, and opens the way for the automated analysis of polar transport at tissue scale.
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