Acute Induction of Translocon-Mediated Ca2+ Leak Protects Cardiomyocytes Against Ischemia/Reperfusion Injury

Al-Mawla, Ribal; Ducrozet, Mallory; Tessier, Nolwenn; Païta, Lucille; Pillot, Bruno; Gouriou, Yves; Villedieu, Camille; Harhous, Zeina; Paccalet, Alexandre; CROLA DA SILVA, Claire; Ovize, Michel; Bidaux, Gabriel; Ducreux, Sylvie; Van Coppenolle, Fabien

Acute Induction of Translocon-Mediated Ca2+ Leak Protects Cardiomyocytes Against Ischemia/Reperfusion Injury

2020

During myocardial infarction, dysregulation of Ca 2+ homeostasis between the reticulum, mitochondria, and cytosol occurs in cardiomyocytes and leads to cell death. Ca 2+ leak channels are thought to be key regulators of the reticular Ca 2+ homeostasis and cell survival. The present study aimed to determine whether a particular reticular Ca 2+ leak channel, the translocon, also known as translocation channel, could be a relevant target against ischemia/reperfusion-mediated heart injury. To achieve this objective, we first used an intramyocardial adenoviral strategy to express biosensors in order to assess Ca 2+ variations in freshly isolated adult mouse cardiomyocytes to show that translocon is a functional reticular Ca 2+ leak channel. Interestingly, translocon activation by puromycin mobilized a ryanodine receptor (RyR)-independent reticular Ca 2+ pool and did not affect the excitation-concentration coupling. Second, puromycin pretreatment decreased mitochondrial Ca 2+ content and slowed down the mitochondrial permeability transition pore (mPTP) opening and the rate of cytosolic Ca 2+ increase during hypoxia. Finally, this translocon pre-activation also protected cardiomyocytes after in vitro hypoxia reoxygenation and reduced infarct size in mice submitted to in vivo ischemia-reperfusion. Altogether, our report emphasizes the role of translocon in cardioprotection and highlights a new paradigm in cardioprotection by functionally uncoupling the RyR-dependent Ca 2+ stores and translocon-dependent Ca 2+ stores.

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