Butyric fermentation: metabolic behaviour and performance of Clostridium tyrobutyricum in a continuous culture with cell recycle
1990
Michel-Savin, D. | Marchal, R. | Vandecasteele, J.P.
Two physiological characteristics of butyric fermentation, inhibition by the acids produced, butyrate and acetate, and dependence on the growth rate of the distribution of these acids, prompted a study of butyrate production in a continuous fermentation system with cell recycle by microfiltration. The influence of the main operating parameters, glucose input (feed concentration and dilution rate) and bleed dilution rate on production of acids and biomass was studied. The performance of the system greatly exceeded the results obtained in batch and simple continuous fermentations as a high productivity for butyrate (9.5 g l–1 h–1) was achieved whilst retaining a satisfactory concentration of butyrate (29.7 g l–1) and low acetate production (0.6 g l–1) at a cell biomass concentration of 35 g l–1. Cell growth rate was found to be a critical parameter for performance stability as oscillations in metabolic activity due to inhibition by acids were observed at bleed dilution rates below 0.016 h–1.
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