Apolipoprotein(a) Inhibits Hepatitis C Virus Entry Through Interaction With Infectious Particles
2017
Oliveira, Catarina | Fournier, Carole | Descamps, Veronique | Morel, Virginie | Scipione, Corey A. | Romagnuolo, Rocco | Koschinsky, Marlys L. | Boullier, Agnes | Marcelo, Paulo | Domon, Jean-Marc | Brochot, Etienne | Duverlie, Gilles | Francois, Catherine | Castelain, Sandrine | Helle, Francois | Unité de Virologie clinique et fondamentale (UVCF) ; Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie | UFR Langues, Cultures et Communication - Clermont-Auvergne (UFR LCC - UCA) ; Université Clermont Auvergne [2017-2020] (UCA [2017-2020]) | Aramco Research & Innovation | Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV) ; Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie | Spectrométrie de Masse Biologique et Protéomique (USR3149 / FRE2032) (SMBP) ; Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) | Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI) ; Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro) ; Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL) | SFR Condorcet ; Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS) | Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ) ; Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie | Hémostase et Remodelage Vasculaire Post-Ischémie (HERVI - EA 3801) ; Université de Reims Champagne-Ardenne (URCA) | Centre Hospitalier Universitaire de Reims (CHU Reims)
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Show more [+] Less [-]English. The development of different cell culture models has greatly contributed to increased understanding of the hepatitis C virus (HCV) life cycle. However, it is still challenging to grow HCV clinical isolates in cell culture. If overcome, this would open new perspectives to study HCV biology, including drug-resistant variants emerging with new antiviral therapies. In this study we hypothesized that this hurdle could be due to the presence of inhibitory factors in patient serum. Combining polyethylene glycol precipitation, iodixanol gradient, and size-exclusion chromatography, we obtained from HCV-seronegative sera a purified fraction enriched in inhibitory factors. Mass spectrometric analysis identified apolipoprotein(a) (apo[a]) as a potential inhibitor of HCV entry. Apo(a) consists of 10 kringle IV domains (KIVs), one kringle V domain, and an inactive protease domain. The 10 KIVs are present in a single copy with the exception of KIV type 2 (KIV2), which is encoded in a variable number of tandemly repeated copies, giving rise to numerous apo(a) size isoforms. In addition, apo(a) covalently links to the apolipoprotein B component of a low-density lipoprotein through a disulfide bridge to form lipoprotein(a). Using a recombinant virus derived from the JFH1 strain, we confirmed that plasma-derived and recombinant lipoprotein(a) as well as purified recombinant apo(a) variants were able to specifically inhibit HCV by interacting with infectious particles. Our results also suggest that small isoforms are less inhibitory than the large ones. Finally, we observed that the lipoprotein moiety of HCV lipoviroparticles was essential for inhibition, whereas functional lysine-binding sites in KIV7, KIV8, and KIV10 were not required. Conclusions: Our results identify apo(a) as an additional component of the lipid metabolism modulating HCV infection.
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