Ecotoxicoproteomics: A decade of progress in our understanding of anthropogenic impact on the environment
2019
Gouveia, Duarte | Almunia, Christine | Cogne, Yannick | Pible, Olivier | Degli Esposti, Davide | Salvador, Arnaud | Cristobal, Susana | Sheehan, David | Chaumot, Arnaud | Geffard, Olivier | Armengaud, J. | Laboratoire Innovations technologiques pour la Détection et le Diagnostic (LI2D) ; Service de Pharmacologie et Immunoanalyse (SPI) ; Médicaments et Technologies pour la Santé (MTS) ; Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS) ; Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | RiverLy (UR Riverly) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | ISA-ANABIO-MS - Analyse biomoléculaire par spectrométrie de masse - Biological Analysis by Mass Spectrometry ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) | Dept Clin & Expt Med, Cell Biol ; Linköping University (LIU) | Ikerbasque - Basque Foundation for Science | We thank the "Commissariat a l'Energie Atomique et aux Energies Alternatives" (France), the "Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture" (France), the "Institut des Sciences Analytiques" (France), and the ANR program "ProteoGam" (ANR-14-CE21-0006-02) for financial support. We also thank Rossana Simas for graphical assistance with the figuress. | ANR-14-CE21-0006,ProteoGam,Protéomique pour de nouveaux biomarqueurs en écotoxicologie chez les gammares: challenge de la biodiversité et immunoanalyse multiplexée comme outil de diagnostic(2014)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA [ADD1_IRSTEA]Systèmes aquatiques soumis à des pressions multiples
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Show more [+] Less [-]English. Anthropogenic pollutants are found worldwide. Their fate and effects on human and ecosystem health must be appropriately monitored. Today, ecotoxicology is focused on the development of new methods to assess the impact of pollutant toxicity on living organisms and ecosystems. In situ biomonitoring often uses sentinel animals for which, ideally, molecular biomarkers have been defined thanks to which environmental quality can be assessed. In this context, high-throughput proteomics methods offer an attractive approach to study the early molecular responses of organisms to environmental stressors. This approach can be used to identify toxicity pathways, to quantify more precisely novel biomarkers, and to draw the possible adverse outcome pathways. In this review, we discuss the major advances in ecotoxicoproteomics made over the last decade and present the current state of knowledge, emphasizing the technological and conceptual advancements that allowed major breakthroughs in this field, which aims to "make our planet great again". SIGNIFICANCE: Ecotoxicoproteomics is a protein-centric methodology that is useful for ecotoxicology and could have future applications as part of chemical risk assessment and environmental monitoring. Ecotoxicology employing non-model sentinel organisms with highly divergent phylogenetic backgrounds aims to preserve the functioning of ecosystems and the overall range of biological species supporting them. The classical proteomics workflow involves protein identification, functional annotation, and extrapolation of toxicity across species. Thus, it is essential to develop multi-omics approaches in order to unravel molecular information and construct the most suitable databases for protein identification and pathway analysis in non-model species. Current instrumentation and available software allow relevant combined transcriptomic/proteomic studies to be performed for almost any species. This review summarizes these approaches and illustrates how they can be implemented in ecotoxicology for routine biomonitoring.
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