Molecular strategies for adapting <em>Bacillus subtilis</em> 168 biosurfactant production to biofilm cultivation mode
2019
Bruck, Hannah Luise | Delvigne, Frank | Dhulster, Pascal | Jacques, Philippe | Coutte, François | Université de Liège | Institut Charles Viollette (ICV) - EA 7394 (ICV) ; Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille | Toulouse Biotechnology Institute (TBI) ; Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) | Agroressources et Impacts environnementaux (AgroImpact) ; Institut National de la Recherche Agronomique (INRA) | ALIBIOTECH program ; University of Liège ; Hauts-de-France Région
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Show more [+] Less [-]English. Biofilm bioreactors have already been proven to be efficient systems for microbial lipopeptide production since they avoid foam formation. However, the cell adhesion capacities of the laboratory strain B. subtilis 168 to the biofilm bioreactor support are limited. In this work, we present a novel approach for increasing cell adhesion through the generation of filamentous and/or exopolysaccharide producing B. subtilis 168 mutants by genetic engineering. The single cell growth behavior was analyzed using time-lapse microscopy and the colonization capacities were investigated under continuous flow conditions in a drip-flow reactor. Cell adhesion could be increased three times through filamentous growth in lipopeptide producing B. subtilis 168 derivatives strains. Further restored exopolysaccharide production increased up to 50 times the cell adhesion capacities. Enhanced cell immobilization resulted in 10 times increased surfactin production. These findings will be of particular interest regarding the design of more efficient microbial cell factories for biofilm cultivation.
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