Actin nucleation at the centrosome controls lymphocyte polarity.
2015
Obino, Dorian | Farina, Francesca | Malbec, Odile | Sáez, Pablo J | Maurin, Mathieu | Gaillard, Jérémie | Dingli, Florent | Loew, Damarys | Gautreau, Alexis | Yuseff, Maria-Isabel | Blanchoin, Laurent | Théry, Manuel | Lennon-Duménil, Ana-Maria | Immunité et cancer (U932) ; Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) | Laboratoire de physiologie cellulaire végétale (LPCV) ; Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Laboratoire de Spectrométrie de Masse Protéomique ; Institut Curie [Paris] | Laboratoire de Biochimie de l'Ecole polytechnique (BIOC) ; École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS) | Pontificia Universidad Católica de Chile (UC) | research grant from FONDECYT No. 1141182 | ANR-12-BSV3-0014,PoLyBEX,Regulation de la réponse humorale par la polarité cellulaire et l'exocytose(2012) | ANR-12-BSV5-0004,MITOTUBES,Disséquer la Dynamique des Microtubules en Mitose par Reconstitution de Sous-Modules du Fuseau(2012) | European Project: 243103,EC:FP7:ERC,ERC-2009-StG,STRAPACEMI(2009) | European Project: 310472,EC:FP7:ERC,ERC-2012-StG_20111109,SPICY(2012)
International audience
Show more [+] Less [-]English. Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark of cell polarity is the reorientation of the centrosome that allows repositioning of organelles and vesicles in an asymmetric fashion. The mechanisms underlying centrosome polarization are not fully understood. Here we found that in resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which is needed for centrosome tethering to the nucleus via the LINC complex. Upon lymphocyte activation, Arp2/3 is partially depleted from the centrosome as a result of its recruitment to the immune synapse. This leads to a reduction in F-actin nucleation at the centrosome and thereby allows its detachment from the nucleus and polarization to the synapse. Therefore, F-actin nucleation at the centrosome-regulated by the availability of the Arp2/3 complex-determines its capacity to polarize in response to external stimuli.
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