Investigation of Aspergillus fumigatus biofilm formation by various “omics” approaches
2013
Muszkieta, Laetitia | Beauvais, Anne | Pähtz, Vera | Gibbons, John | Le Berre, Véronique | Beau, Rémi | Shibuya, Kazutoshi | Rokas, Antonis | François, Jean Marie | Kniemeyer, Olaf | Brakhage, Axel | Latgé, Jean | Aspergillus ; Institut Pasteur [Paris] (IP) | Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany] | Vanderbilt University [Nashville] | Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) ; Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) | Toho University | Department of Biological Sciences [Nashville] ; Vanderbilt University [Nashville] | Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute) | European Union ; HEALTH-2010-260338 ERA-Net ; ESF (European science Foundation) Fuminomics
International audience
Show more [+] Less [-]English. In the lung, Aspergillus fumigatus usually forms a dense colony of filaments embedded in a polymeric extracellular matrix called biofilm (BF). This extracellular matrix embeds and glues hyphae together and protects the fungus from an outside hostile environment. This extracellular matrix is absent in fungal colonies grown under classical liquid shake conditions (PL), which were historically used to understand A. fumigatus pathobiology. Recent works have shown that the fungus in this aerial grown BF-like state exhibits reduced susceptibility to antifungal drugs and undergoes major metabolic changes that are thought to be associated to virulence. These differences in pathological and physiological characteristics between BF and liquid shake conditions suggest that the PL condition is a poor in vitro disease model. In the laboratory, A. fumigatus mycelium embedded by the extracellular matrix can be produced in vitro in aerial condition using an agar-based medium. To provide a global and accurate understanding of A. fumigatus in vitro BF growth, we utilized microarray, RNA-sequencing, and proteomic analysis to compare the global gene and protein expression profiles of A. fumigatus grown under BF and PL conditions. In this review, we will present the different signatures obtained with these three "omics" methods. We will discuss the advantages and limitations of each method and their complementarity.
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