Differential expression of the efflux pumps p-glycoprotein and multidrug resistance-associated protein in human monocyte-derived dendritic cells | Expression différentielle des pompes d'efflux p-glycoprotein et de la mulitdrug resistance-associated protein dans les cellules dendritiques dérivées de monocytes
2001
Laupèze, B | Amiot, L | Bertho, Nicolas | Grosset, J.M | Lehne, G | Fauchet, R | Fardel, O | Centre Hospitalier Universitaire de Rennes [CHU Rennes] = Rennes University Hospital [Pontchaillou]
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Show more [+] Less [-]English. P-glycoprotein (P-gp), an ATP-binding cassette (ABC) drug efflux pump, has been recently shown to play an important role in the physiology of Langherans cells, a subtype of dendritic cells (DC) found in the skin. The present study was designed to investigate expression and activity of P-gp and of multidrug resistance-associated protein (MRP), another ABC efflux pump sharing numerous substrates with P-gp, in human monocytederived DC. Immunolabeling experiments and dye efflux assays indicated that such cells displayed elevated levels of MRP activity and expression when compared to those present in parental monocytes. Generation of DC from monocytes in the presence of the MRP inhibitor indomethacin did not, however, alter the capacity of DC to stimulate allogeneic T cells proliferation in mixed lym-phocyte reaction. In addition, indomethacin did not inhibit the up-regulation of the CD1a, a marker occurring during the differentiation of monocytes into DC. In contrast to that of MRP, functional expression of P-gp was not detected in monocyte-derived DC. Such antigen presenting cells that constitute a promising tool for antitumor vaccinal therapy therefore display differential expression of the efflux pumps P-gp and MRP. Human Immunology 62, 1073-1080 (2001).
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