Dynamics of antibiotic resistance genes and presence of putative pathogens during ambient temperature anaerobic digestion
2014
Alves Resende, Juliana | Diniz, C.G. | Silva, V.L. | Otenio, M H | Bonnafous, Anaïs | Arcuri, P.B. | Godon, Jean-Jacques | Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE) ; Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences ; Federal University of Juiz de Fora (UFIJ) | Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa) | Secretariat for International Relations ; Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa) | EMBRAPA's virtual Laboratory abroad (Labex Program) in Europe, Brazilian National Council for Scientific and Technological Development (CNPq), Research Support Foundation of Minas Gerais (FAPEMIG), Coordination of Improvement of the Personnel of Higher Education (CAPES) (11853-12-3)
This study was focused on evaluating the persistency of antimicrobial resistance (AR) genes and putative pathogenic bacteria in an anaerobic digesters operating at mesophilic ambient temperature, in two different year seasons: summer and winter.[br/]Abundance and dynamic of AR genes encoding resistance to macrolides (ermB), aminoglycosides (aphA2) and beta-lactams (blaTEM -1 ) and persistency of potentially pathogenic bacteria in pilot-scale anaerobic digesters were investigated. AR genes were determined in the influent and effluent in both conditions. Overall, after 60 days, reduction was observed for all evaluated genes. However, during the summer, anaerobic digestion was more related to the gene reduction as compared to winter. Persistency of potentially pathogenic bacteria was also evaluated by metagenomic analyses compared to an in-house created database. Clostridium, Acinetobacter and Stenotrophomonas were the most identified.[br/]Overall, considering the mesophilic ambient temperature during anaerobic digestion (summer and winter), a decrease in pathogenic bacteria detection through metagenomic analysis and AR genes is reported. Although the mesophilic anaerobic digestion has been efficient, the results may suggest medically important bacteria and AR genes persistency during the process.[br/]This is the first report to show AR gene dynamics and persistency of potentially pathogenic bacteria through metagenomic approach in cattle manure ambient temperature anaerobic digestion.
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