The Root-Colonizing Endophyte Piriformospora indica Supports Nitrogen-Starved Arabidopsis thaliana Seedlings with Nitrogen Metabolites
2023
Scholz, Sandra, S | Barth, Emanuel | Clément, Gilles | Marmagne, Anne | Ludwig-Müller, Jutta | Sakakibara, Hitoshi | Kiba, Takatoshi | Vicente-Carbajosa, Jesús | Pollmann, Stephan | Krapp, Anne | Oelmüller, Ralf | Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany] | Institut Jean-Pierre Bourgin - Sciences du végétal (IJPB) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Technische Universität Dresden = Dresden University of Technology (TU Dresden) | Graduate School of Bioagricultural Sciences ; Nagoya University | Universidad Politécnica de Madrid (UPM) | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA) | ICPS EIG CONCERT-Japan | 01DR17007A and 01DR17007B from the Federal Ministry of Education and Research (BMBF), Germany | EIG_JC1JAPAN-045 from the Centre National de la Recherche Scientifique (CNRS),France | PCIN-2016–037 from the Ministry of Economy and Competitiveness (MINECO), Spain | JPMJSC16C3 from the Japan Science and Technology Agency (JST) | Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) | CRC1127 ChemBioSys (project ID: 239748522) | Saclay Plant Sciences-SPS (ANR-17-EUR-0007) | ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017)
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Show more [+] Less [-]English. The root-colonizing endophytic fungus Piriformospora indica promotes the root and shoot growth of its host plants. We show that the growth promotion of Arabidopsis thaliana leaves is abolished when the seedlings are grown on media with nitrogen (N) limitation. The fungus neither stimulated the total N content nor did it promote 15NO3− uptake from agar plates to the leaves of the host under N-sufficient or N-limiting conditions. However, when the roots were co-cultivated with 15N-labelled P. indica, more labels were detected in the leaves of N-starved host plants but not in plants supplied with sufficient N. Amino acid and primary metabolite profiles, as well as the expression analyses of N metabolite transporter genes suggest that the fungus alleviates the adaptation of its host from the N limitation condition. P. indica alters the expression of transporter genes, which participate in the relocation of NO3−, NH4+ and N metabolites from the roots to the leaves under N limitation. We propose that P. indica participates in the plant’s metabolomic adaptation against N limitation by delivering reduced N metabolites to the host, thus alleviating metabolic N starvation responses and reprogramming the expression of N metabolism-related genes.
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