Influence of adjuvant formulation on the induced protection of mice immunized with total soluble antigen of Trichinella spiralis
2004
Deville, Sebastien | de Pooter, Ambre | Aucouturier, Jérome | Laine-Prade, Veronique | Cote, Martine | Boireau, Pascal | Vallee, Isabelle | Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques ; Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | Société d'Exploitation de Produits pour les Industries Chimiques (SEPPIC)
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Show more [+] Less [-]English. Vaccination of pigs against the helminth nematode Trichinella could be a good alternative to prevent the risk of human infection. In order to develop an efficient and safe vaccine, the choice of the adjuvant is an important issue. In this study, two adjuvants were selected to prepare vaccines based on total soluble Trichinella spiralis muscle larvae (ML) antigen: Montanide (R) ISA70 water in oil emulsion and Montanide (R) IMS nanoparticles. Aluminium hydroxide was used as a reference adjuvant. The immune response was checked by ELISA of parasite antigen specific IgG1 and IgE. Finally, protection induced in vaccinated mice was measured after a T spiralis challenge by counting ML burdens. The results clearly showed an impact of adjuvants on the specific IgG1 and IgE antibody responses against T spiralis. Differences were observed between the rates of protection induced according to the type of formulation, although the three adjuvants tested were able to enhance the humoral immune response. This work demonstrated the need to use an adjuvant to obtain a specific IgG1 and IgE responses directed against the total soluble extract of T spiralis.
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