Comparative genomics of extrachromosomal elements in Bacillus thuringiensis subsp. israelensis
2017
Bolotine, Alexandre | Gillis, Annika | Sanchis Borja, Vincent | Nielsen-Leroux, Christina | Mahillon, Jacques | Lereclus, Didier | Sorokine, Alexei | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Laboratory of Food and Environmental Microbiology ; Earth and Life Institute [Louvain-La-Neuve] (ELI) ; Université Catholique de Louvain = Catholic University of Louvain (UCL)-Université Catholique de Louvain = Catholic University of Louvain (UCL) | Université Catholique de Louvain = Catholic University of Louvain (UCL) | ANR-12-ADAP-0018,PathoBactEvol,Evolution du pouvoir pathogène d'une bactérie opportuniste sporulante dans l'environnement(2012)
Bacillus thuringiensis subsp. israelensis is one of the most important microorganisms used against mosquitoes. It was intensively studiedfollowing its discovery and became a model bacterium of the B. thuringiensis species. Those studies focused on toxin genes, aggregationassociatedconjugation, linear genome phages, etc. Recent announcements of genomic sequences of different strains have not been explicitlyrelated to the biological properties studied. We report data on plasmid content analysis of four strains using ultra-high-throughput sequencing.The strains were commercial product isolates, with their putative ancestor and type B. thuringiensis subsp. israelensis strain sequenced earlier.The assembled contigs corresponding to published and novel data were assigned to plasmids described earlier in B. thuringiensis subsp.israelensis and other B. thuringiensis strains. A new 360 kb plasmid was identified, encoding multiple transporters, also found in most of theearlier sequenced strains. Our genomic data show the presence of two toxin-coding plasmids of 128 and 100 kb instead of the reported 225 kbplasmid, a co-integrate of the former two. In two of the sequenced strains, only a 100 kb plasmid was present. Some heterogeneity exists in thesmall plasmid content and structure between strains. These data support the perception of active plasmid exchange among B. thuringiensis subsp.israelensis strains in nature.
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