A global benchmark study using affinity-based biosensors
2009
Rich, Rebecca L. | Papalia, Giuseppe A. | Flynn, Peter J. | Furneisen, Jamie | Quinn, John | Klein, Joshua S. | Katsamba, Phini S. | Waddell, M. Brent | Scott, Michael | Thompson, Joshua | Berlier, Judie | Corry, Schuyler | Baltzinger, Mireille | Zeder-Lutzi, Gabrielle | Schoenemann, Andreas | Clabbers, Anca | Wieckowski, Sébastien | Murphy, Mary M. | Page, Phillip | Ryan, Thomas E. | Duffner, Jay | Ganguly, Tanmoy | Corbin, John | Gautam, Satyen | Anderluh, Gregor | Bavdek, Andrej | Reichmann, Dana | Yadav, Satya P. | Hommema, Eric | Pol, Ewa | Drake, Andrew | Klakamp, Scott | Chapman, Trevor | Kernaghan, Dawn | Miller, Ken | Schuman, Jason | Lindquist, Kevin | Herlihy, Kara | Murphy, Michael B. | Bohnsack, Richard | Andrien, Bruce | Brandani, Pietro | Terwey, Danny | Millican, Rohn | Darling, Ryan J. | Wang, Liann | Carter, Quincy | Dotzlaf, Joe | Lopez-Sagaseta, Jacinto | Campbell, Islay | Torreri, Paola | Hoos, Sylviane | England, Patrick | Liu, Yang | Abdiche, Yasmina | Malashock, Daniel | Pinkerton, Alanna | Wong, Melanie | Lafer, Eileen | Hinck, Cynthia | Thompson, Kevin | Di Primo, Carmelo | Joyce, Alison | Brooks, Jonathan | Torta, Federico | Hagel, Anne Birgitte Bagge | Krarup, Janus | Pass, Jesper | Ferreira, Monica | Shikov, Sergei | Mikolajczyk, Malgorzata | Abe, Yuki | Barbato, Gaetano | Giannetti, Anthony M. | Krishnamoorthy, Ganeshram | Beusink, Bianca | Satpaev, Daulet | Tsang, Tiffany | Fang, Eric | Partridge, James | Brohawn, Stephen | Horn, James | Pritsch, Otto | Obal, Gonzalo | Nilapwar, Sanjay | Busby, Ben | Gutierrez-Sanchez, Gerardo | das Gupta, Ruchira | Canepa, Sylvie | Witte, Krista | Nikolovska-Coleska, Zaneta | Cho, Yun Hee | d'Agata, Roberta | Schlick, Kristian | Calvert, Rosy | Munoz, Eva M. | Hernaiz, Maria Jose | Bravman, Tsafir | Dines, Monica | Yang, Min-Hsiang | Puskas, Agnes | Boni, Erica | Li, Jiejin | Wear, Martin | Grinberg, Asya | Baardsnes, Jason | Dolezal, Olan | Gainey, Melicia | Anderson, Henrik | Peng, Jinlin | Lewis, Mark | Spies, Peter | Trinh, Quyhn | Bibikov, Sergei | Raymond, Jill | Yousef, Mohammed | Chandrasekaran, Vidya | Feng, Yuguo | Emerick, Anne | Mundodo, Suparna | Guimaraes, Rejane | Mcgirr, Katy | Li, Yue-Ji | Hughes, Heather | Mantz, Hubert | Skrabana, Rostislav | Witmer, Mark | Ballard, Joshua | Martin, Loïc, M | Skladal, Petr | Korza, George | Laird-Offringa, Ite | Lee, Charlene S. | Khadir, Abdelkrim | Podlaski, Frank | Neuner, Phillippe | Rothacker, Julie | Rafique, Ashique | Dankbar, Nico | Kainz, Peter | Gedig, Erk | Vuyisich, Momchilo | Boozer, Christina | Ly, Nguyen | Toews, Mark | Uren, Aykut | Kalyuzhniy, Oleksandr | Lewis, Kenneth | Chomey, Eugene | Pak, Brian J. | Myszka, David G. | School of Medicine [University of Utah, Salt Lake City] ; University of Utah | KaloBios Pharmaceuticals [San Francisco] | Schering–Plough Biopharma [Palo Alto] | Nomadics [Oklahoma City] | Biochemistry and Molecular Biophysics [Pasadena] ; California Institute of Technology (CALTECH) | Department of Biochemistry and Molecular Biophysics ; Columbia University [New York] | Hartwell Center for Bioinformatics and Biotechnology [Memphis] ; St Jude Children's Research Hospital [Memphis, USA] | Universität Zürich [Zürich] = University of Zurich (UZH) | Molecular Probes/Invitrogen [Eugene] | Institut de génétique et biologie moléculaire et cellulaire (IGBMC) ; Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | MerckKGaA ; Merck Sharp and Dohme (Merck & Co. Inc) | Abbott Bioresearch Center [Worcester] | Institut de biologie moléculaire et cellulaire (IBMC) ; Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) | Reichert [Depew} | Momenta Pharmaceuticals [Cambridge] | XOMA (U.S.) | Department of Chemical and Biomolecular Engineering, National University of Singapore ; National University of Singapore (NUS) | Department of Biology [Ljubljiana] ; University of Ljubljana | Weizmann Institute of Science [Rehovot, Israël] | Molecular Biotechnology Core Laboratory [Cleveland] ; Cleveland Clinic Foundation | ThermoFisher Scientific [Rockford] | Biacore/GE Healthcare [Uppsala] | AstraZeneca [Hayward] | Neurodegeneration Research Department [Harlow] ; GlaxoSmithKline [Harlow] | MedImmune | Biacore/GE Healthcare [Piscataway] | Department of Biochemistry [Milwaukee] ; Medical College of Wisconsin [Milwaukee] (MCW) | Alexion Pharmaceuticals [Cheshire] | Applied Biosystems [Foster City] | diaDexus [South San Francisco] | Eli Lilly and Company [Indianapolis] | Center for Applied Medical Research [Plamplona] (CIMA) ; Universidad de Navarra [Pamplona] (UNAV) | EMD Lexigen Research Center [Billerica] | Department of Cell Biology and Neuroscience [Rome] ; Istituto Superiore di Sanità = National Institute of Health (ISS) | Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry ; Institut Pasteur [Paris] (IP) | Department of Chemistry [Atlanta] ; Georgia State University ; University System of Georgia (USG)-University System of Georgia (USG) | Pfizer/Rinat Laboratories [South San Francisco] | PDLBioPharma [Fremont] | Department of Biochemistry [San Antonio] ; University of Texas Health Science Center at San Antonio [San Antonio, Tx, USA] | Akubio [Cambridge] | ARN : régulations naturelle et artificielle ; Université Bordeaux Segalen - Bordeaux 2-Institut Européen de Chimie et de Biologie-Institut National de la Santé et de la Recherche Médicale (INSERM) | Wyeth Research [Cambridge] | Department of Biochemistry and Molecular Biology [Odense] ; University of Southern Denmark (SDU) | NovoNordisk [Gentofte] | Södertörns University College [Huddinge] | Department of Biochemistry [Philadelphia] ; Temple University [Philadelphia] ; Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE) | U.S. Food and Drug Administration (FDA) | Department of Biochemical Engineering [London] ; University College of London [London] (UCL) | Merck [Rome] | Roche [Palo Alto] | University of Twente | Agensys [Santa Monica] | Novartis [Emeryville] | Massachusetts Institute of Technology (MIT) | Northern Illinois University | Institut Pasteur de Montevideo ; Pasteur Network (Réseau International des Instituts Pasteur) | University of Manchester [Manchester] | Department of Physiology [Baltimore] ; University of Maryland [Baltimore] (UMB) | Complex Carbohydrate Research Center [Athens, GA, USA] ; University of Georgia [USA] | Adnexus Therapeutics [Waltham} | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) | ForteBio [Menlo Park] | University of Michigan [Ann Arbor] ; University of Michigan System | Human Genome Sciences [Rockville] | Department of Chemical Sciences [Catania] ; Università degli studi di Catania = University of Catania (Unict) | Montana State University (MSU) | GKT School of Biomedical Sciences [London] | Organic and Pharmaceutical Chemistry Department [Madrid] ; Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM) | Bio-Rad Haifa | Institute of Chemistry [Taipei] ; Academia Sinica | Department of Microbiology and Molecular Genetics ; The University of Texas Health Science Center at Houston (UTHealth) | Fred Hutchinson Cancer Research Center [Seattle] (FHCRC) | Division of Molecular Structure [London] ; National Institute of Medical Research (UK) | Centre for Translational and Chemical Biology, Institute of Structural and Molecular Biology [Edinburgh] ; The University of Edinburgh | Acceleron Pharma [Cambridge] | Biotechnology Research Institute [Montreal] ; National Research Council of Canada (NRC) | CSIRO Health Sciences and Nutrition [Parkville] ; Commonwealth Scientific and Industrial Research Organisation [Australia] (CSIRO) | Battelle Biomedical Research Center [Columbus] | Attana AB [Stockholm] | Corning Incorporated | School of Life Sciences, Institute for Chemistry and Bioanalytics [Muttenz] ; Fachhochschule Nordwestschweiz [Windisch, Schweiz] = FHNW University of Applied Sciences and Arts Northwestern Switzerland (FHNW) | Bio-Rad [Hercules] | Monsanto [Chesterfield] | Genzyme [Cambridge] | Universität des Saarlandes [Saarbrücken] = Saarland University [Saarbrücken] | Institute of Neuroimmunology of SAS [Bratislava] | Bristol-Myers Squibb [Princeton] | Array Biopharma [Boulder] | Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS) ; Médicaments et Technologies pour la Santé (MTS) ; Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Masaryk University [Brno] = Masarykova univerzita [Brno] = Université Masaryk [Brno] (MU / MUNI) | University of Connecticut (UCONN) | University of Southern California (USC) | GeminX Biotechnologies [Montreal] | Hoffman–La Roche [Nutley] | IRBM, Pomezia [Rome] | Ludwig Institute for Cancer Research | Regeneron Pharmaceuticals [Tarrytown, NY] | University of Muenster | Department of Molecular Biology [Salzburg] ; University of Salzburg | XanTec Bioanalytics [Muenster] | Biosciences Division [Los Alamos] ; Los Alamos National Laboratory (LANL) | Lumera [Bothell] | Arizona State University [Tempe] (ASU) | Dyax [Cambridge] | Lombardi Comprehensive Cancer Center ; Georgetown University [Washington] (GU) | Department of Biochemistry [Seattle] ; University of Washington [Seattle] | ZymoGenetics | Bio-Rad Canada [Edmonton] | Bio-Rad Canada [Toronto]
International audience
Show more [+] Less [-]English. To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. (C) 2008 Elsevier Inc. All rights reserved.
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