Glycerol is not suitable for the cryopreservation of quail semen
2024
Grasseau, Isabelle | Couty, Isabelle | Grimaud-Jottreau, Isabelle | Vitorino Carvalho, Anais | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Unité Expérimentale Avicole de Tours (UE PEAT) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This work was supported by the Agence Nationale de la Recherche [ANR-11-INBS-0003]. | ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011)
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Show more [+] Less [-]English. This study investigated the potential use of glycerol as a cryoprotectant for quail sperm cells. Its role in maintaining sperm fertilising ability in vivo and in vitro quality parameters, such as motility, was assessed.The data showed that the presence of glycerol in semen samples was associated with infertility, which suggested that removal prior to insemination is mandatory. Removal through serial dilution centrifugation was associated with fewer than 5% of motile sperm cells and resulted in no fertility.In conclusion, glycerol alone is not suitable for quail semen cryopreservation, and other approaches need to be investigated to develop cryobanking programmes for this species.
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