Serum bile acids profiles are altered without change of the gut microbiota composition following a seven-day prednisolone therapy in severe alcoholic hepatitis
2024
Esparteiro, Damien | Fouquet, Grégory | Courtois, Anoïsia | Jedraszak, Guillaume | Marticho, Léa | Gourdel, Mathilde | Billon-Crossouard, Stéphanie | Croyal, Mikaël | Naassila, Mickaël | Nguyen-Khac, Eric | Marcq, Ingrid | Lallemand SAS | Microbiologie Environnement Digestif Santé (MEDIS) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Groupe de Recherche sur l'alcool et les pharmacodépendances - UMR INSERM_S 1247 UPJV (GRAP) ; Université de Picardie Jules Verne (UPJV)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Unité de génétique médicale et oncogénétique [CHU Amiens Picardie] ; CHU Amiens-Picardie | HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM) ; Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM) | Structure fédérative de recherche François Bonamy (Nantes Univ - SFR François Bonamy) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Nantes Université (Nantes Univ) | Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes) | ITX-lab unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX-lab) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) | Centre de Recherche en Nutrition Humaine Ouest (CRNH Ouest) | CHU Amiens-Picardie
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Show more [+] Less [-]English. Severe Alcoholic Hepatitis (sAH) is an acute form of liver injury caused by chronic and heavy alcohol drinking. A one-month corticosteroids course is the only sAH reference treatment, and its interactions with the Gut Microbiota (GM), which is a key contributor to liver injury, remain unknown. To evaluate the evolution of the GM in sAH patients, we retrospectively investigated the composition of the GM of 27 sAH patients at the Amiens University Hospital before (D0) and after (D7) a 7-day corticotherapy course using fecal metagenomics sequencing. We also quantified fecal Short-Chain Fatty Acids (SCFA) and fecal and serum Bile Acids (BA), as well as serum Lipopolysaccharide-Binding Protein (LBP). Overall, the community and taxonomical analyses did not reveal any GM evolution between D0 and D7, nor did the SCFA profiles analysis. However, in serum but not fecal samples, the ratio of glyco-conjugated to tauro-conjugated BA was significantly reduced at D7, independently of the response to treatment, while two BA were enriched in non-responder patients. LBP concentration significantly diminished between D0 and D7, which may indicate an improvement of the gut barrier. The stability of the GM of sAH is interesting in the perspective of new treatments based on GM modulation.
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