Evolutionary novelty in the apoptotic pathway of aphids
2020
Ribeiro Lopes, Mélanie | Parisot, Nicolas | Gaget, Karen | Huygens, Cissy | Peignier, Sergio | Duport, Gabrielle | Orlans, Julien | Charles, Hubert | Baatsen, Pieter | Jousselin, Emmanuelle | da Silva, Pedro | Hens, Korneel | Callaerts, Patrick | Calevro, Federica | Biologie Fonctionnelle, Insectes et Interactions (BF2I) ; Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven) | Leuven Center for Cancer Biology (VIB-KU-CCB) ; Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven)-Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB) | Centre de Biologie pour la Gestion des Populations (UMR CBGP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Oxford Brookes University | This work was supported by the Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; INSA (Institut National des Sciences Appliquees), Lyon; the French ANR-16-CE02-0014 (HMicMac) program grant; and KU Leuven grant C14/17/099. INSA-Lyon supported P.C. with an Invited Professorship. | ANR-16-CE02-0014,Hmicmac,Co-adaptations hôtes-microbiote: mécanismes et conséquences(2016)
International audience
Show more [+] Less [-]English. Apoptosis, a conserved form of programmed cell death, shows interspecies differences that may reflect evolutionary diversification and adaptation, a notion that remains largely untested. Among insects, the most speciose animal group, the apoptotic pathway has only been fully characterized in Drosophila melanogaster, and apoptosis-related proteins have been studied in a few other dipteran and lepidopteran species. Here, we studied the apoptotic pathway in the aphid Acyrthosiphon pisum, an insect pest belonging to the Hemiptera, an earlier-diverging and distantly related order. We combined phylogenetic analyses and conserved domain identification to annotate the apoptotic pathway in A. pisum and found low caspase diversity and a large expansion of its inhibitory part, with 28 inhibitors of apoptosis (IAPs). We analyzed the spatiotemporal expression of a selected set of pea aphid IAPs and showed that they are differentially expressed in different life stages and tissues, suggesting functional diversification. Five IAPs are specifically induced in bacteriocytes, the specialized cells housing symbiotic bacteria, during their cell death. We demonstrated the antiapoptotic role of these five IAPs using heterologous expression in a tractable in vivo model, the Drosophila melanogaster developing eye. Interestingly, IAPs with the strongest antiapoptotic potential contain two BIR and two RING domains, a domain association that has not been observed in any other species. We finally analyzed all available aphid genomes and found that they all show large IAP expansion, with new combinations of protein domains, suggestive of evolutionarily novel aphidspecific functions.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique