Cytotoxicity control of silicon nanoparticles by biopolymer coating and ultrasound irradiation for cancer theranostic applications
2017
Sviridov, a P | Osminkina, L A | Kharin, Alexander | Gongalsky, M B | Kargina, J V | Kudryavtsev, a A | Bezsudnova, Yu I | Perova, T S | Geloen, Alain | Lysenko, V | Timoshenko, V Yu | Department of Physiology ; Universidade de São Paulo = University of São Paulo (USP) | International Laboratory BioNanophoton ; National Research Nuclear University | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN) ; Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM) | InstituteTheoretical and Experiment Biophysics ; the Russian Academy of Sciences [Moscow, Russia] (RAS) | Department of Electrical and Electronic Engineering ; Okayama University | ITMO Univiversity | International Laboratory BioNanophoton ; National Research Nuclear University | Centre National de la Recherche Scientifique (CNRS) | INL - Spectroscopies et Nanomatériaux (INL - S&N) ; Institut des Nanotechnologies de Lyon (INL) ; École Centrale de Lyon (ECL) ; Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL) ; Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) | Institut des Nanotechnologies de Lyon (INL) ; École Centrale de Lyon (ECL) ; Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) | Russian Science Foundation 16-13-10145; 'Andre Mazon' program; INSA de Lyon
Silicon nanoparticles (SiNPs) prepared by mechanical grinding of luminescent porous silicon were coated with a biopolymer (dextran) and investigated as a potential theranostic agent for bioimaging and sonodynamic therapy. Transmission electron microscopy, photoluminescence and Raman scattering measurements of dextran-coated SiNPs gave evidence of their enhanced stability in water. In vitro experiments confirmed the. lower cytotoxicity of the dextran-coated NPs. in comparison with uncoated ones, especially for. high concentrations of about 2 mg ml(-1). Efficient uptake of the NPs. by cancer cells was found using bioimaging in the optical transmittance and photoluminescence modes. Treatment. of the cells with uptaken SiNPs by therapeutic ultrasound for 5-20 min resulted in a strong decrease in. the number of. living cells, while the total number of cells remained nearly unchanged. The obtained data indicate a ` mild' effect of the combined action of ultrasonic irradiation. and SiNPs on cancer cells. The observed results reveal new opportunities for. controlling the photoluminescent and sonosensitizing properties of silicon-based NPs. for applications in the diagnostics and mild therapy of cancer.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique
