Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal
2021
Guesmi, Sihem | Pujic, Petar | Nouioui, Imen | Dubost, Audrey | Najjari, Afef | Ghedira, Kais | Igual, José | Miotello, Guylaine | Cherif, Ameur | Armengaud, Jean | Klenk, Hans-Peter | Normand, Philippe | Sghaier, Haïtham | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Universite Lyon 1 UMR 5557Ecologie Microbienne (France) National Center for Nuclear Sciences and Technologies CNSTN (MESRS, Tunisia) BIODESERT research program (BVBGR, ISBST, University of Manouba (MESRS, Tunisia) LR11-ES31LABGeM (CEA/Genoscope CNRS) UMR8030 | ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010) | ANR-11-INBS-0013,IFB (ex Renabi-IFB),Institut français de bioinformatique(2011)
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Show more [+] Less [-]English. A new strain belonging to the genus Kocuria, designed PT10, was isolated from irradiated roots of the xerophyte Panicum turgidum. Isolate PT10 is a Gram-positive, coccoid, aerobic and ionizing-radiation (IR)-resistant actinobacterium. PT10 has shown an ability to survive under extreme conditions, such as gamma irradiation, desiccation and high concentration of hydrogen peroxide. Phenotypic, chemotaxonomic and comparative genome analyses support the assignment of strain PT10 (LMG 31102 = DSM 108617) as Kocuria rhizophila. The complete genome sequence of PT10 consists of one chromosome (2,656,287 bps), with a 70.7% G + C content and comprises 2481 protein-coding sequences. A total of 1487 proteins were identified by LC-MS/MS profiling. In silico analyses revealed that the proteome of the oxidation-tolerant PT10 possesses several features explaining its IR-resistant phenotype and many adaptive pathways implicated in response to environmental pressures - desiccation, cold, reactive oxygen species and other stressors.
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