Highly Sensitive Voltammetric Catechol Biosensor Based on Electroaddressing of Laccase Encapsulated in Modified Chitosan
2020
Galai, Hajer Chakroun | Rahmouni, Nihed | Namour, Philippe | Bonhomme, Anne | Bessueille, François | Hentati, Salma Besbes | Jaffrezic-Renault, Nicole | Laboratory of Materials Chemistry (LR13ES08) ; Faculté des Sciences de Bizerte [Université de Carthage] ; Université de Carthage (Tunisie) (UCAR)-Université de Carthage (Tunisie) (UCAR) | ISA-Surfaces ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | RiverLy - Fonctionnement des hydrosystèmes (RiverLy) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ISA-Micro & Nanobiotechnologies ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)
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Show more [+] Less [-]English. In this work, a new voltammetric biosensor, based on the encapsulation of laccase from Trametes versicolor in an electrodeposited nanocomposite film, was developed for the detection of catechol. The nanocomposite was composed of chitosan (CS) modified with trymiristine (Tr), both of natural origin, to enhance its conductivity. FTIR and SEM studies were performed to characterize the modified chitosan film and the encapsulation of the enzyme. The analytical performance of the elaborated biosensor was determined through voltammetry detection of the produced quinone. Catechol was detected in a linear range between 10–20 and 10–15 M and a sensitivity of 0.4667 mA/p [catechol] was found. The repeatability of this biosensor is good as its RSD is equal to 1.35% and it shows stability for four weeks. To test the functionality of the developed biosensor, the total phenolic content of three samples of natural oils was determined and compared to a colorimetric test.
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