Standardized protocol for determination of biohydrogen potential
2020
Carrillo-Reyes, Julian | Buitron, German | Moreno-Andrade, Iván | Tapia-Rodríguez, Aida Cecilia | Palomo-Briones, Rodolfo | Razo-Flores, Elías | Aguilar-Juárez, Oscar | Arreola-Vargas, Jorge | Bernet, Nicolas | Braga, Adriana Ferreira Maluf | Braga, Lucia | Castelló, Elena | Chatellard, Lucile | Etchebehere, Claudia | Fuentes, Laura | León-Becerril, Elizabeth | Mendez-Acosta, Hugo Oscar | Ruiz-Filippi, Gonzalo | Tapia-Venegas, Estela | Trably, Eric | Wenzel, Jorge | Zaiat, Marcelo | Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM) | Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT) ; Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT) | Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ) ; Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT) | Universidad Tecnologica de Jalisco (UTJ) | Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Universidade de São Paulo = University of São Paulo (USP) | Universidad de la República [Montevideo] (UDELAR) | Instituto de Investigaciones Biológicas Clemente Estable [Montevideo] (IIBCE) | Departamento de Ingeniería Química [Guadalajara] ; Universidad de Guadalajara | Universidad de Guadalajara | Pontificia Universidad Católica de Valparaíso (PUCV) | Escuela de Ingeniería Bioquímica [Valparaíso] ; Pontificia Universidad Católica de Valparaíso (PUCV) | Escola de Engenharia de São Carlos (EESC-USP) ; Universidade de São Paulo = University of São Paulo (USP) | Fondo de Sustentabilidad Energética SENER−CONACYT, Clúster Biocombustibles Gaseosos, 247006 and CONACYT240087 | Fondecyt 3160219 | Program ECOS-CONICYT project N° C12E06, GRAIL613667 (FP7-KBBE-2013) | NII-FSE102488 (Uruguay) | FAPESP (2015/06246-7) | CNPq processes 406751/2013-7, 150641/2015-0, 150475/2016-0 (Brazil) | European Project: 326974,EC:FP7:PEOPLE,FP7-PEOPLE-2012-IEF,WASTE2BIOHY(2013)
International audience
Show more [+] Less [-]English. Biohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 different countries, to assess the biohydrogen potential. As quality criteria, a coefficient of variation of the cumulative hydrogen production (Hmax) was defined to be <15 %. Two options to run BHP batch tests were proposed; a manual protocol with periodic measurements of biogas production, needing conventional laboratory materials and analytical equipment for biogas characterization; and an automatic protocol, which is run in a device developed for online measurements of low biogas production. The detailed procedures for both protocol options are presented, as well as data validating them. The validation showed acceptable repeatability and reproducibility, measured as intra- and inter-laboratory coefficient of variation, which can be reduced up to 9 %.
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