MALDI mass spectrometry imaging (MSI) as a new and promising method to investigate polysaccharides in wheat grains
2013
Velickovic, Dusan | Ropartz, David, D. | Durand, Sylvie | Saulnier, Luc | Guillon, Fabienne | Rogniaux, Hélène | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de la Recherche Agronomique (INRA) | Institut National de Recherche Agronomique (INRA). UR Biopolymères, Interactions Assemblages (1268).
National audience
Show more [+] Less [-]English. Matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry emerged recently as a new imaging method with outstanding features. It provides a means of resolving both the spatial distribution of many kinds of molecules and their molecular structure in tissue sections. After preparing tissue section and coating with an appropriate MALDI matrix, the instrument captures a series of mass spectra, each of which represents the mass profile of a specific region in the sample [1]. Sensitivity, high information content and rapidity of tissue analysis, in addition to the fact that no previous knowledge is required on the monitored molecules, may give MSI tremendous advantage over other imaging techniques (e.g. microscopy with immune-labelling). In this work, MSI was used to monitor the distribution and chemical variability of arabinoxylans (AX) and β-glucans (BG) in cross sections of different development stages of Triticum aestivum L. These two polysaccharides are the main cell wall components of wheat endosperm and their structure have a significant effect on wheat grain development as well as on the end-uses and functional properties of the grain [2]. To the authors’ best knowledge, this study is the first attempt to image cell wall polysaccharides in intact tissue by MS. The presentation will highlight some unique results obtained on developing wheat grains.
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