Revisiting plant plasma membrane lipids in tobacco: a focus on sphingolipids
2016
Cacas, Jean-Luc | Bure, Corinne | Grosjean, Kévin | Gerbeau Pissot, Patricia | Lherminier, Jeannine | Rombouts, Yoann | Maes, Emmanuel | Bossard, Claire | Gronnier, Julien | Furt, Fabienne | Fouillen, Laetitia | Germain, Véronique | Bayer, Emmanuelle | Cluzet, Stéphanie | Robert, Franck | Schmitter, Jean-Marie | Deleu, Magali | Lins, Laurence | Simon Plas, Françoise | Mongrand, Sébastien | Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement | Laboratoire de biogenèse membranaire (LBM) ; Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) | Centre National de la Recherche Scientifique (CNRS) | Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF) ; Université de Lille-Centre National de la Recherche Scientifique (CNRS) | Laboratoire de Biophysique Moléculaire aux Interfaces ; TERRA Teaching and Research Centre ; Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] ([FUSAGx]) ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] ([FUSAGx]) ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège) | Worcester Polytechnic Institute | Université de Bordeaux (UB) | Bordeaux Metabolome Facility-MetaboHUB [ANR-11-INBS-0010] ; ARC FIELD project Finding Interesting Elicitor Lipids ; Fonds Speciaux pour la Recherche, University of Liege ; Belgian Funds for Scientific Research ; Tres Grande Infrastructure de Recherche-Resonance Magnetique Nucleaire-Tres Hauts Champs Centre National de la Recherche Scientifique [Fr3050] | ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011)
International audience
Show more [+] Less [-]English. The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 +/- 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed.
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique
