NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety
2019
Obeid, Sameh | Sung, Pei-Shan | Le Roy, Benoit | Chou, Ming-Li | Hsieh, Shie-Liang | Elie-Caille, Celine | Burnouf, Thierry | Boireau, Wilfrid | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Université Bourgogne Franche-Comté [COMUE] (UBFC) | Academia Sinica | Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering ; Taipei Medical University | Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering ; Taipei Medical University | International PhD Program in Biomedical Engineering, College of Biomedical Engineering ; Taipei Medical University | ANR-17-CE09-0025,MADNESS,Une approche microfluidique générique pour la qualification des nanoparticules biologiques(2017)
Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs).We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (TPEVs)can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVseffective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and nonnanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (N 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.
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