Topical bioassay of Oberon® on Drosophila melanogaster pupae: delayed effects on ovarian proteins, cuticular hydrocarbons and sexual behaviour
2021
Bensafi-Gheraibia, Hanene | Kissoum, Nesrine | Hamida, Zahia Cirine | Farine, Jean-Pierre | Soltani, Noureddine | Université Badji Mokhtar [Annaba] = Badji Mokhtar University [Annaba] = (عنابة) جامعة باجي مختار–عنابة (UBMA) | Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA) ; Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | The National Fund for Scientific Research of Algeria and the Ministry of Higher Education and Scientific Research of Algeria (PRFU project D01N01UN230120190009).
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Show more [+] Less [-]English. Spiromesifen (Oberon? 240 SC; concentrated solution; Bayer CropScience, Germany) a pesticide derived from spirocyclic tetronic acids, acts mainly though blocking lipid biosynthesis via acetyl-CoA carboxylase. However, its mode of action requires further analyses. This study aims to evaluate the sublethal effects of spiromesifen on the fruit fly Drosophila melanogaster. The treatment was carried out by topical application on newly moulted pupae using two concentrations (LD10 = 21.45 and LD25 = 39.53 ?g a.i./pupa). Our results showed that the developmental duration of larvae and pupae were not significantly affected by treatment. However, spiromesifen caused delayed effects on flies that survived from treated pupae. Spiromesifen was found to decrease significantly both the morphometric measurements and the protein contents of ovaries. The protein pattern of ovaries showed a reduction in the number of protein bands affecting the progeny via vitellogenesis. Finally, compared to controls, spiromesifen decreased significantly the amounts of some cuticular hydrocarbons in treated female and male adults. Consequently, in response to the changes in cuticular hydrocarbons, their sexual behaviour was also affected. Our study brings new insights about the multiple effects and physiological consequences of disturbing lipid metabolism.
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