A limited set of transcriptional programs define major cell types
2020
Breschi, Alessandra | Muñoz-Aguirre, Manuel | Wucher, Valentin | Davis, Carrie | Garrido-Martín, Diego | Djebali, Sarah | Gillis, Jesse | Pervouchine, Dmitri | Vlasova, Anna | Dobin, Alexander | Zaleski, Chris | Drenkow, Jorg | Danyko, Cassidy | Scavelli, Alexandra | Reverter, Ferran | Snyder, Michael P. | Gingeras, Thomas R. | Guigo, Roderic | Barcelona Institute of Science and Technology (BIST) | Universitat Pompeu Fabra [Barcelona] (UPF) | Stanford University | Universitat Politècnica de Catalunya = Université polytechnique de Catalogne [Barcelona] (UPC) | Cold Spring Harbor Laboratory (CSHL) | Institut de Recherche en Santé Digestive (IRSD) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Skolkovo Institute of Science and Technology [Moscow] (Skoltech) | Research Institute of Molecular Pathology (IMP) | United States Department of Health & Human Services | National Institutes of Health (NIH) - USA (U54HG007004) | NIH National Human Genome Research Institute (NHGRI) (U41HG007234) | National Human Genome Research Institute of the National Institutes of Health (R01MH101814) | Spanish Ministry of Economy and Competitiveness, Centro de Excelencia Severo Ochoa 2013-2017 (SEV-20120208) | Programa de Ayudas FPI del Ministerio de Economia y Competitividad(BES-2012-055848) | Ministerio de Educacion, Cultura y Deporte, under the FPU programme (Formacion de Profesorado Universitario) (FPU15/03635) | CERCA programme/Generalitat de Catalunya | "la Caixa"-Severo Ochoa predoctoral fellowship (LCF/BQ/SO15/52260001) | Common Fund of the Office of the Director of the National Institutes of Health | European Project: 294653,EC:FP7:ERC,ERC-2011-ADG_20110310,RNA-MAPS(2012)
International audience
Show more [+] Less [-]English. We have produced RNA sequencing data for 53 primary cells from different locations in the human body. The clustering of these primary cells reveals that most cells in the human body share a few broad transcriptional programs, which define five major cell types: epithelial, endothelial, mesenchymal, neural, and blood cells. These act as basic components of many tis-sues and organs. Based on gene expression, these cell types redefine the basic histological types by which tissues have been traditionally classified. We identified genes whose expression is specific to these cell types, and from these genes, we estimat-ed the contribution of the major cell types to the composition of human tissues. We found this cellular composition to be a characteristic signature of tissues and to reflect tissue morphological heterogeneity and histology. We identified changes in cellular composition in different tissues associated with age and sex, and found that departures from the normal cellular composition correlate with histological phenotypes associated with disease.
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