A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs
2019
Cho, In-Cheol | Park, Hee-Bok | Ahn, Jin Seop | Han, Sang-Hyun | Lee, Jae-Bong | Lim, Hyun-Tae | Yoo, Chae-Kyoung | Jung, Eun-Ji | Kim, Dong-Hwan | Sun, Wu-Sheng | Ramayo-Caldas, Yuliaxis | Kim, Sang-Geum | Kang, Yong-Jun | Kim, Yoo-Kyung | Shin, Hyun-Sook | Seong, Pil-Nam | Hwang, In-Sul | Park, Beom-Young | Hwang, Seongsoo | Lee, Sung-Soo | Ryu, Youn-Chul | Lee, Jun-Heon | Ko, Moon-Suck | Lee, Kichoon | Andersson, Göran | Pérez-Enciso, Miguel | Lee, Jeong-Woong | National Institute of Animal Science | Kongju National University | Korea Research Institute of Bioscience & Biotechnology (KRIBB) | Jeju National University | Chonbuk National University | Gyeongsang National University | Bio-Medical Science Co | University of Science and Technology | Génétique Animale et Biologie Intégrative (GABI) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA) | Chungnam National University (CNU) | The Ohio State University Press | Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Centre for Research in Agricultural Genomics (CRAG) | Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona (UAB) | Institució Catalana de Recerca i Estudis Avançats = Catalan Institution for Research and Advanced Studies (ICREA) | Cooperative Research Program for Agriculture Science Technology Development (Project title: A research on performance improvement of `Nanchukmacdon', a high quality pig breed developed using Korean native black pigs (KNP) in Jeju Island" Rural Developme PJ01012301 PJ01262701 | Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program, Republic of Korea | Ministerio de Economia y Competitividad, Spain AGL2016-78709-R
International audience
Show more [+] Less [-]English. Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.
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