A DEMETER-like DNA demethylase governs tomato fruit ripening
2015
Liu, Ruie | How-Kit, Alexandre | Stammitti, Linda | Teyssier, Emeline | Rolin, Dominique, D. | Mortain-Bertrand, Anne | Halle, Stephanie | Liu, Mingchun | Kong, Junhua | Wu, Chaoqun | Degraeve-Guilbault, Charlotte | Chapman, Natalie H | Maucourt, Mickael, M. | Hodgman, T Charlie | Tost, Jorg | Bouzayen, Mondher | Hong, Yiguo | Seymour, Graham B | Giovannoni, James J | Gallusci, Philippe | Biologie du fruit et pathologie (BFP) ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) | Fondation Jean Dausset - Centre d’Étude du Polymorphisme Humain | Génomique et Biotechnologie des Fruits (GBF) ; Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse (ENSAT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Hangzhou Normal University | University of Nottingham, UK (UON) | Boyce Thompson Institute [Ithaca] | ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011)
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Show more [+] Less [-]English. In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening- an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.
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