Influence of nutrient, pH and dissolved oxygen on the production of Metarhizium flavoviride Mf189 blastospores in submerged batch culture
2005
Issaly, N. | Chauveau, H. | Aglevor, F. | Fargues, Jacques, J. | Durand, Alain | Centre de ressources technologiques Agro-Alimentaire et Bio-Industriel (SAYENS 2ABI) (CRT SAYENS 2ABI) ; SATT SAYENS [Dijon] | Office of International Research and Development ; Partenaires INRAE | Centre de Biologie pour la Gestion des Populations (UMR CBGP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Microbiologie ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)
Corresponding author. fax: +33-3-8069-3229.E-mail address: [email protected] (A. Durand).
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Show more [+] Less [-]English. The influence of different parameters on the sporulation of Metarhizium flavoviride was studied during submerged cultures in shake flasks and in 5 l bioreactors. The screening in shake flasks of several carbon and nitrogen sources allowed the definition of an optimal medium, based on sucrose and brewer’s yeast with a C/N ratio of 1.6. With this medium, a production of 5.4 _ 108 blastospores per ml (Bspores ml_1) was obtained after 169 h of cultivation. The influence of pH and pO2 was independently studied in 5 l working volume bioreactors using the optimal medium. The best production was obtained with pH and pO2 regulated respectively to 7 and 100%. Finally, when the culture was grown under optimized conditions, the blastospores concentration increased 16-fold, with 1.1 109 Bspores ml-1 obtained after 144 h cultivation. This represents a gain of productivity of about 4.8 times
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