A maize MuDR-like transposable element transcribed in the rice genome
2003
Yoshida, S. | Asakura, N. | Ootani, R. | Nakamura, C.
We previously showed that proline added to the subculture medium dramatically increased the frequency of albino plants in rice tissue culture under high osmotic conditions. Using the differential display method, a proline-induced transcript was identified that showed considerable homology to the maize mudrA transcript of the MuDR transposon. We obtained 12 genomic clones, of which two clones, OsMu4-2 (4.7 kb) having terminal inverted repeats (TIRs) and OsMu10-1 (1.1 kb) with highly homologous TIRs, were analyzed. Both had 9-bp putative target-site duplications outside of the TIRs. RT-PCR products from the coding region of OsMu4-2 were obtained using primers designed by the genomic sequence. The structural analysis suggested that OsMuDR is a rice orthologue of the maize MuDR. An amino acid sequence of the MURA-like protein deduced from OsMu4-2, however, included several stop codons in the reading frame. Results suggested that OsMu4-2 was a pseudogene. OsMu10-1 possessed only a 330-bp sequence of the 3'-end of the coding region. This deleted version possessed 5-nucleotide direct repeats at the deletion point, suggesting that the deletion was caused by the interrupted gap repair mechanism. The presence of such a deletion provided an indication that the rice MuDR element had transposed in the past.
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