In vitro conservation of Ruta angustifolia Pers.
1997
Kristina, N.N. | Seswita, D. (Balai Penelitian Tanaman Rempah dan Obat, Bogor (Indonesia))
English. In vitro germplasm conservation is focused on endangered species of medicinal crops. These kinds of crops are usually conserved in collection garden, with high risk of losing by disease infection or bad condition of climate. Ruta angustifolia is classified as endangered species. In vitro conservation has been conducted by maintaining a small part of plant on MS media applied with growth retardant (ABA and Manitol) or by maintaining a growing culture with repeated subculture. Conservation in culture of ABA 4 mg/l and manitol 1000 mg/l gave viability regeneration for six months. Conservation on the growing media of 3/4 MS + BA 0.1 mg/l with subculture after three months gave constant viability after four years of subculture
Show more [+] Less [-]unknown. Pelestarian plasma nutfah secara in vitro diutamakan pada tumbuhan obat langka. Inggu (Ruta angustifolia) dikategorikan sebagai tanaman yang keberadaannya di alam sudah langka. Penyimpanan secara in vitro telah dilakukan dengan menggunakan tunas yang ditanam pada media MS ditambah zat penghambat tumbuh (ABA dan Manitol) maupun dalam keadaan tumbuh yang disubkultur setiap tiga bulan secara berulang. Penyimpanan di dalam kultur menggunakan ABA 5 mg/l dan manitol 1000 mg/l memberikan daya regeneratif yang tetap selama enam bulan. Penyimpanan dalam keadaan tumbuh yang disubkultur setiap tiga bulan sekali, pada media 3/4 MS + BA 0.1 mg/l memberikan daya regenerasi yang tetap setelah disubkultur selama empat tahun
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