Potensi protein kecambah kedelai dalam menstimulasi sekresi insulin pada pankreas tikus normal dan diabetes | Potential of germinated soybean protein to simulate insulin secretion in the pancreas of normal and diabetic rats

and centrifuged The supernatant was stored at -20 deg. C for determination of insulin. The insulin analysis was conducted by ELISA method. The results showed that soluble protein profile and the insulin secretion of the islets. The germinated SP has the ability to stimulate insulin secretion of the pancreas of diabetic as well as normal rats. The insulin secretion of the islets in the medium of crude TI of germinated soybean (GPTI) was the highest. The potential order of the insulin secretion of normal rats islets were GPTI, KTI, BBI, GPI

English. The objective of this investigation was to examine the potential of seed and germinated SP that were extracted at two condition precipitation, Kunitz Trypsin Inhibitor (KTI) and Bowman Birk Inhibitor (BBI) to stimulate insulin secretion of the pancreas of induced diabetic and normal rats by in vitro bio assay. Mature male Sprague Dawley (SD) rats of the in vitro bio assay were divided into two groups. They were diabetic rats that was induced by aloksan injection and normal rats (without aloksan injection). Rat was anesthetized with ether

and the pancreas was taken out, injected by HBS solution, hydrolyzed by collagenase and then washed several times with RP M1 I 640 LITE solution and Kreb Ringer bicarbonate buffer before incubation. The islets of the pancreas were incubated in Kreb buffer under seven conditions of media treatment as follows: glucose medium that was used as a reference standard (R), total protein of seed (SPT), total protein of germinated soybean (GPT), TI protein of seed (SPTI), TI protein of germinated soybean (GPTI), KTI, and BBI. After two hours of incubation, the mature was sonicated

SPTI, BBI, KTI, SPT and R media. The potential of germinated SP to stimulate insulin secretion was better than ungerminated SP.

and R media. While the potential order of the insulin secretion of diabetic rat islets were GPTI, GPI

SPTI, SPT