The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis
2020
Smith, Stephanie | Zhu, Shanshuo | Joos, Lisa | Roberts, Ianto | Nikonorova, Natalia | Vu, Lam Dai | Stes, Elisabeth | Cho, Hyunwoo | Larrieu, Antoine | Xuan, Wei | Goodall, Benjamin | van de Cotte, Brigitte | Waite, Jessic Marie | Rigal, Adeline | Harborough, Sigurd Ramans | Persiau, Geert | Vanneste, Steffen | Kirschner, Gwendolyn K. | Vandermarliere, Elien | Martens, Lennart | Stahl, Yvonne | Audenaert, Dominique | Friml, Jiri | Felix, Georg | Simon, Ruediger | Bennett, Malcolm J. | Bishopp, Anthony | De Jaeger, Geert | Ljung, Karin | Kepinski, Stefan | Robert, Stephanie | Nemhauser, Jennifer | Hwang, Ildoo | Gevaert, Kris | Beeckman, Tom | De Smet, Ive
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabi-lizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.
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