Cloning of double stranded DNA coding for hordein polypeptides [messenger RNA, reverse transcription, molecular cloning]
1979
Brandt, A.
Double stranded cDNA was synthesized from a partially purified hordein mRNA preparation isolated from developing barley endosperm. The double stranded cDNA was inserted by homopolymeric tailing into the Hind III site of plasmid pBR322. Following bacterial transformation one recombinant plasmid which contains an 850 base pair insert was shown to hybridize to the mRNAs coding for two of the B-hordein polypeptides
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