Transcription products associated with developing pea chloroplast membranes [Pisum sativum, light induced transcription, plastid DNA, differential gene activity, developing plastids, membrane associated polysomes, RNA]
1980
Stumann, B.M. | Kaufmann, U. | Henningsen, K.W. (Kongelige Veterinaer- og Landbohoejskole, Copenhagen (Denmark). Dept. of Genetcis)
RNA fractions from leaves of pea (Pisum sativum L.) were labelled by placing dark-grown shoots in ('32)P-phosphate for 1 h in darkness and 3 h in light. Membrane-associated RNA and soluble RNA was isolated from an organelle fraction containing plastids and mitochondria. A cytoplasmic RNA fraction was also isolated. These RNA fractions were hybridized to nuclear, chloroplast or mitochondrial DNA isolated from fully green leaves. The organelle RNA fractions hybridized singificantly only to chloroplast DNA. This result suggests that at early stages of greening, light initiates transcription of part of the chloroplast DNA. Some of the newly transcribed RNA species are associated with the developing plastid membranes
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