Isolation of two Rodoturula rubra strains showing differences in the degradation of aromatic compounds
Wright, J.D. | Ratledge, C.
Two strains of Rhodotorula rubra, isolated by enrichment from soil contaminated with oil using 4-hydroxybenzoate as sole carbon and energy source, grew only on benzenoid compounds hydroxylated in the 4-position. Both yeasts contained two inducible NADH-requiring hydroxylase enzymes, one specific for 4-hydroxybenzoate and the second specific for 3, 4-dihydroxybenzoate. Additionally, intradiol ring-cleavage enzymes for both 3,4-dihydroxybenzoate and 1,3, 4-trihydroxybenzene were detected in cell-free extracts of the strains. Although the thermal stability and pH optima of the ring-cleavage enzymes differ between the two yeasts, both yeasts have been established as simultaneously possessing both ring-cleavage activities, which has hitherto not been reported.Show more [+] Less [-]